Anti-Human PTCH2 Rabbit Recombinant Antibody

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  FC experiment of SH-SY5Y using 98705-2-RR

  1x10^6 SH-SY5Y cells were surface stained with 0.25 ug Anti-Human PTCH2 Rabbit RecAb (98705-2-RR, Clone: 251786C3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98705-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98705-2-RR against Human PTCH2 were performed. The affinity constant is below 1 pM.

PTCH2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98705-2-RR

Clone No.251786C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Patched 2, patched homolog 2 (Drosophila), Protein patched homolog 2, PTC2, UNQ560/PRO1121/PRO57079

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Tested Applications

• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98705-2-RR targets PTCH2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: patched homolog 2 (Drosophila)
• Calculated Molecular Weight: 131 kDa
• GenBank Accession Number: NM_003738
• Gene Symbol: PTCH2
• Gene ID (NCBI): 8643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6C5
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2.