|Positive WB detected in||mouse testis tissue, rat testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11213-1-AP targets Nectin-3/PVRL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Nectin-3/PVRL3 fusion protein Ag1712|
|Full Name||poliovirus receptor-related 3|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||70-80 kDa|
|GenBank accession number||BC017572|
|Gene ID (NCBI)||25945|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cancer Commun (Lond)
ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.
Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.
Characterization of nectin processing mediated by presenilin-dependent γ-secretase.
Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.
Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Morgan (Verified Customer) (08-09-2021)
This PVRL3 antibody worked wonderfully with 2 ovarian cancer cell lines; OV8 and OV90. I was very pleased with the results.