Nectin-3/PVRL3 Polyclonal antibody

Nectin-3/PVRL3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11213-1-AP

Synonyms

CD113, CDw113, nectin 3, Nectin-3, poliovirus receptor related 3, PPR3, PRR3, PVRL3, PVRR3



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11213-1-AP targets Nectin-3/PVRL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin-3/PVRL3 fusion protein Ag1712
Full Name poliovirus receptor-related 3
Calculated molecular weight 61 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC017572
Gene symbol PVRL3
Gene ID (NCBI) 25945
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Nectin-3/PVRL3 antibody 11213-1-APDownload protocol
IHC protocol for Nectin-3/PVRL3 antibody 11213-1-APDownload protocol
IP protocol for Nectin-3/PVRL3 antibody 11213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biol Reprod

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.

Authors - Przemysław Zakrzewski
humanIHC

Biomed Pharmacother

Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.

Authors - Feixue Xu
mouse,ratWB,IF

J Neurochem

Characterization of nectin processing mediated by presenilin-dependent γ-secretase.

Authors - Kim Jinsook J
humanWB

Front Microbiol

Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.

Authors - Dennis Schöttelndreier
humanWB

Cancer Commun (Lond)

ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.

Authors - Yin Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Morgan (Verified Customer) (08-09-2021)

This PVRL3 antibody worked wonderfully with 2 ovarian cancer cell lines; OV8 and OV90. I was very pleased with the results.

  • Applications: Western Blot
  • Cell Tissue Type: OV8 and 0V90
Nectin-3/PVRL3 Antibody Western Blot validation ( dilution) in OV8 and 0V90 (Cat no:11213-1-AP)