|Positive WB detected in
|mouse testis tissue, rat testis tissue
|Positive IP detected in
|mouse testis tissue
|Positive IHC detected in
|human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11213-1-AP targets Nectin-3/PVRL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Nectin-3/PVRL3 fusion protein Ag1712
|poliovirus receptor-related 3
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Cancer Commun (Lond)
ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.
Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.
Characterization of nectin processing mediated by presenilin-dependent γ-secretase.
Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.
Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Morgan (Verified Customer) (08-09-2021)
This PVRL3 antibody worked wonderfully with 2 ovarian cancer cell lines; OV8 and OV90. I was very pleased with the results.