Validation Data Gallery
|Positive WB detected in||HEK-293 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:100|
|Sample-dependent, check data in validation data gallery|
66674-1-Ig targets PARK2/Parkin in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||PARK2/Parkin fusion protein Ag5179|
|Full Name||Parkinson disease (autosomal recessive, juvenile) 2, parkin|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||42-52 kDa|
|GenBank accession number||BC022014|
|Gene ID (NCBI)||5071|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
J Pineal Res
Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics
CircEPS15, as a sponge of MIR24-3p ameliorates neuronal damage in Parkinson disease through boosting PINK1-PRKN-mediated mitophagy
Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates.
Oxid Med Cell Longev
Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue
Oxid Med Cell Longev
Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway.
Cell Death Discov
Stomatin-like protein 2 deficiency exacerbates adverse cardiac remodeling
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.