PARK2/Parkin Monoclonal antibody

PARK2/Parkin Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2H5A7

Cat no : 66674-1-Ig

Synonyms

AR JP, LPRS2, PARK2, Parkin, Parkinson disease protein 2, PDJ, PRKN



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:100
Sample-dependent, check data in validation data gallery

Product Information

66674-1-Ig targets PARK2/Parkin in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PARK2/Parkin fusion protein Ag5179
Full Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated molecular weight 52 kDa
Observed molecular weight 42-52 kDa
GenBank accession numberBC022014
Gene symbol PARK2
Gene ID (NCBI) 5071
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
IHC protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
IF protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oxid Med Cell Longev

Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway.

Authors - Xue Wang
mouseWB

Life Sci

Hypoxic training upregulates mitochondrial turnover and angiogenesis of skeletal muscle in mice.

Authors - Yong-Cai Zhao
humanWB

J Proteome Res

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Authors - Kun Meng
mouseIF

J Inflamm Res

Resveratrol Relieves Gouty Arthritis by Promoting Mitophagy to Inhibit Activation of NLRP3 Inflammasomes.

Authors - Weimin Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain