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  • KD/KO Validated

PARK2/Parkin Monoclonal antibody

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H5A7

Cat no : 66674-1-Ig

Synonyms

AR JP, LPRS2, PARK2, PARK2/Parkin, Parkin, Parkinson disease protein 2, PDJ, PRKN



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66674-1-Ig targets PARK2/Parkin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PARK2/Parkin fusion protein Ag5179
Full Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 42-52 kDa
GenBank Accession NumberBC022014
Gene Symbol Parkin
Gene ID (NCBI) 5071
RRIDAB_2882028
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
IHC protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
IF protocol for PARK2/Parkin antibody 66674-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
human,mouseWB,IF

Autophagy

CircEPS15, as a sponge of MIR24-3p ameliorates neuronal damage in Parkinson disease through boosting PINK1-PRKN-mediated mitophagy

Authors - Yuanzhang Zhou
mouseWB

Nat Commun

Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates.

Authors - Helena Costa-Verdera
humanIF

Oxid Med Cell Longev

Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway.

Authors - Xue Wang
ratWB

Oxid Med Cell Longev

Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue

Authors - Guo-Jie Han
mouseIF,WB

Cell Death Discov

Stomatin-like protein 2 deficiency exacerbates adverse cardiac remodeling

Authors - Yuntao Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Paula (Verified Customer) (07-26-2024)

Worked well for Western Blotting when made fresh. However, after freezing antibody stock (in 3% BSA) did not work.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Bone Marrow Mesenchymal Stem Cells
PARK2/Parkin Antibody Western Blot validation (1:1000 dilution) in Human Bone Marrow Mesenchymal Stem Cells (Cat no:66674-1-Ig)
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain