Validation Data Gallery
|Positive WB detected in||HEK-293 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:100|
|Sample-dependent, check data in validation data gallery|
66674-1-Ig targets PARK2/Parkin in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||PARK2/Parkin fusion protein Ag5179|
|Full Name||Parkinson disease (autosomal recessive, juvenile) 2, parkin|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||42-52 kDa|
|GenBank accession number||BC022014|
|Gene ID (NCBI)||5071|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
Oxid Med Cell Longev
Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway.
Toxicol Appl Pharmacol
Neurotoxicity of a pyrethroid pesticide deltamethrin is associated with the imbalance in proteolytic systems caused by mitophagy activation and proteasome inhibition.
Hypoxic training upregulates mitochondrial turnover and angiogenesis of skeletal muscle in mice.
J Proteome Res
Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.
SARS-CoV-2 Causes Mitochondrial Dysfunction and Mitophagy Impairment.
J Inflamm Res
Resveratrol Relieves Gouty Arthritis by Promoting Mitophagy to Inhibit Activation of NLRP3 Inflammasomes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.