PARK2/Parkin Monoclonal antibody, PBS Only

PARK2/Parkin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A7

Cat no : 66674-1-PBS

Synonyms

AR JP, LPRS2, PARK2, PARK2/Parkin, Parkin, Parkinson disease protein 2, PDJ, PRKN

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Validation Data Gallery

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  WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66674-1-Ig (same clone as 66674-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66674-1-Ig (same clone as 66674-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 66674-1-Ig (PARK2/Parkin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66674-1-PBS targets PARK2/Parkin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PARK2/Parkin fusion protein Ag5179
• Full Name: Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 42-52 kDa
• GenBank Accession Number: BC022014
• Gene Symbol: PARK2
• Gene ID (NCBI): 5071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.