Phospho-4EBP1 (Thr37) Recombinant monoclonal antibody

• 

  WB analysis using 81812-4-RR

  Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-4EBP1 (Thr37) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 81812-4-RR

Clone No.240535G3

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

4EBP1 (Thr37), 4E-BP1 (Thr37), p-4EBP1, p-4E-BP1, p-4EBP1 (Thr37)

---

Tested Applications

• Positive WB detected in: HEK-293T cells, λ phosphatase treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

81812-4-RR targets Phospho-4EBP1 (Thr37) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Calculated Molecular Weight: 118 aa, 12 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC004459
• Gene Symbol: EIF4EBP1
• Gene ID (NCBI): 1978
• RRID: AB_3670507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13541
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Lipids Health Dis; Imidazole propionate ameliorates lipid metabolism in adipocytes to attenuate high-fat diet-induced obesity via PPAR signaling pathway.; Authors - Caiyu Lin
human	WB	Int J Mol Sci; SCAMP3-Driven Regulation of ERK1/2 and Autophagy Phosphoproteomics Signatures in Triple-Negative Breast Cancer.; Authors - Beatriz M. Morales-Cabán
pig	WB	Front Vet Sci; Reduction in within-litter variation of piglet birth weight through dietary supplementation of 3-hydroxyisobutyric acid in sows.; Authors - Long Che
human	WB	Transl Oncol; Next-generation sequencing in early-stage multiple primary lung cancer: The prognostic significance of genomic accumulation status and BCL2L11del; Authors - Mu-Ting Wang