Product Information
87523-1-PBS targets Phospho-CDK9 (Thr186) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cyclin-dependent kinase 9 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001261 |
| Gene Symbol | CDK9 |
| Gene ID (NCBI) | 1025 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50750 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. Phospho-CDK9 (Thr186) is the active-site-switching modification of the catalytic subunit of P-TEFb (positive transcription elongation factor b). Phosphorylation at threonine 186, located in the T-loop of CDK9, is essential for full kinase activity: it swings the T-loop away from the catalytic cleft, allowing ATP and protein substrates (e.g., the C-terminal domain of RNA polymerase II, DSIF and NELF) to enter and be efficiently phosphorylated. Thus, p-Thr186-CDK9 licenses the elongation step of most protein-coding transcripts and is indispensable for productive mRNA synthesis. (PMID: 39800748)
