Phospho-EGFR (Thr693) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87291-1-RR (same clone as 87291-1-PBS)

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87291-1-RR (Phospho-EGFR (Thr693) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87291-1-PBS in a different storage buffer formulation.

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  Schematic diagram of the phosphorylation sites of EGFR.

  In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

Phospho-EGFR (Thr693) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87291-1-PBS

Clone No.252344B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EGF Receptor (Thr669), p-EGFR (T669), p-EGFR (T693), p-EGFR (Thr669), p-EGFR (Thr693)

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Product Information

87291-1-PBS targets Phospho-EGFR (Thr693) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Observed Molecular Weight: 150-180 kDa
• GenBank Accession Number: NM_005228
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. Phospho-EGFR (Thr693) is a phosphorylated form of the epidermal growth factor receptor (EGFR) at the threonine 693 residue. This phosphorylation event is significant as it is involved in the activation of EGFR signaling pathways. EGFR is known to be a transcriptional co-activator for several cancer-promoting genes, including cyclin D1, nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). (PMID: 34295309) Some scientists call EGFR Thr693 as EGFR Thr669, the site where the signaling peptide is removed.