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  • Western Blot (WB) analysis of various lysates using Phospho-EGFR (Thr693) Recombinant monoclonal antib (87291-1-RR)

    WB analysis using 87291-1-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87291-1-RR (Phospho-EGFR (Thr693) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Schematic diagram of the phosphorylation sites of EGFR.

    Schematic diagram of the phosphorylation sites of EGFR.

    In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

Phospho-EGFR (Thr693) Recombinant monoclonal antibody

Phospho-EGFR (Thr693) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87291-1-RR
Clone No.252344B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EGF Receptor (Thr669), p-EGFR (T669), p-EGFR (T693), p-EGFR (Thr669), p-EGFR (Thr693)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inEGF treated A431 cells

This antibody, also known as EGFR Thr669, is the site where the signal peptide is removed.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87291-1-RR targets Phospho-EGFR (Thr693) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Observed Molecular Weight150-180 kDa
GenBank Accession NumberNM_005228
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. Phospho-EGFR (Thr693) is a phosphorylated form of the epidermal growth factor receptor (EGFR) at the threonine 693 residue. This phosphorylation event is significant as it is involved in the activation of EGFR signaling pathways. EGFR is known to be a transcriptional co-activator for several cancer-promoting genes, including cyclin D1, nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). (PMID: 34295309) Some scientists call EGFR Thr693 as EGFR Thr669, the site where the signaling peptide is removed.

Protocols

Product Specific Protocols
WB protocol for Phospho-EGFR (Thr693) antibody 87291-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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