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CoraLite®555-conjugated Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3N6

Cat no : CL555-80340

Synonyms

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Phospho-Hamartin/TSC1 (Ser (CL555-80340)

    FC experiment of HeLa using CL555-80340

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Phospho-Hamartin/TSC1 (Ser (CL555-80340)

    FC experiment of HeLa using CL555-80340

    1X10^6 HeLa cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

Tested Applications

Positive FC detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80340 targets Phospho-Hamartin/TSC1 (Ser511) in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name tuberous sclerosis 1
Calculated Molecular Weight 130 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_000368
Gene Symbol TSC1
Gene ID (NCBI) 7248
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).