CoraLite® Plus 647-conjugated Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4C7G2

Cat no : CL647-66863

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Phospho-Histone H3 (Ser10)

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Validation Data Gallery

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  FC experiment of HeLa using CL647-66863

  1X10^6 unstimulated or 20 µM Nocodazole treated 24 hours HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Phospho-Histone H3 (Ser10) (CL647-66863, Clone:4C7G2). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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  FC experiment of HeLa using CL647-66863

  1X10^6 HeLa cells untreated (dashed lines) or treated with nocodazole (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human Phospho-Histone H3 (Ser10) (CL647-66863, Clone:4C7G2), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive FC detected in: 20 µM Nocodazole treated 24 hours HeLa cells, nocodazole treated HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66863 targets Phospho-Histone H3 (Ser10) in FC (Intra) applications and shows reactivity with Human, rat, mouse, pig samples.

• Tested Reactivity: Human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: NM_003529
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.