Phospho-TAK1 (Thr187) Polyclonal antibody

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• Phospho-TAK1 (Thr187) Polyclonal antibody (28958-1-AP)

Phospho-TAK1 (Thr187) Polyclonal Antibody for WB, ELISA

Cat No. 28958-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Synonyms

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

• 

  WB analysis using 28958-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28958-1-AP (Phospho-TAK1 (Thr187) antibody) at dilution of 1:5000 incubated 4℃ overnight. The membrane was stripped and reblotted with GAPDH antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28958-1-AP targets Phospho-TAK1 (Thr187) in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 579 aa, 64 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC017715
• Gene Symbol: TAK1
• Gene ID (NCBI): 6885
• RRID: AB_2918222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43318
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825).It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Acta Pharm Sin B; Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway; Authors - Maxwell Duah
chicken	WB	Vet Microbiol; miR-17195 promotes infectious bronchitis virus proliferation and macrophage-mediated inflammation via the PLCβ2-TAK1 axis.; Authors - Zheng Wang
mouse	WB,IHC	Inflammation; MALT1 Knockdown Alleviates Multiorgan Injury and Inflammation Through Inhibiting TAK1/NF-κB Signaling Pathway-Mediated T-Helper 1 and 17 Differentiations in LPS-induced Inflammation.; Authors - Qinghe Huang
human	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human	WB	Biochem Biophys Res Commun; YEATS2/TAK1 axis mediates TGF-β1 driven adaptive resistance to sorafenib in hepatocellular carcinoma.; Authors - Lizhi Bai
human	WB	J Int Med Res; Dual-specificity phosphatase 14 inhibits the growth of cervical cancer cells by regulating the transforming growth factor-beta-activated kinase 1 and NOD-like receptor pyrin domain containing 3 inflammatory pathways.; Authors - Yuanyuan Zhang