Phospho-MKK7 (Ser271/Thr275) Polyclonal antibody, PBS Only

• 

  WB analysis using 29199-1-AP (same clone as 29199-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29199-1-AP (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 29199-1-PBS in a different storage buffer formulation.

Phospho-MKK7 (Ser271/Thr275) Polyclonal Antibody for WB, Indirect ELISA

Cat No. 29199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

29199-1-PBS targets Phospho-MKK7 (Ser271/Thr275) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC038295
• Gene Symbol: MKK7
• Gene ID (NCBI): 5609
• RRID: AB_2918246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14733
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)