Phospho-MKK7 (Ser271/Thr275) Polyclonal antibody

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• Phospho-MKK7 (Ser271/Thr275) Polyclonal antibody (29199-1-AP)

Phospho-MKK7 (Ser271/Thr275) Polyclonal Antibody for WB, ELISA

Cat No. 29199-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MAP2K7 (Ser271/Thr275), PRKMK7

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  WB analysis using 29199-1-AP

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29199-1-AP (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29199-1-AP targets Phospho-MKK7 (Ser271/Thr275) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC038295
• Gene Symbol: MKK7
• Gene ID (NCBI): 5609
• RRID: AB_2918246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14733
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)

Publications

What published studies show

The Phospho-MKK7 (Ser271/Thr275) Polyclonal antibody (Cat# 29199-1-AP) has 2 citations. One appears in Cell Reports in Medicine, studying FBXO3-mediated DUSP9 ubiquitination in leukemia stem cell maintenance and tyrosine kinase inhibitor resistance in chronic myeloid leukemia. The other is in Journal of Ethnopharmacology, investigating Xinlikang capsule's effects on cancer-related fatigue, oxidative stress, JNK activation, and bone marrow apoptosis in tumor-bearing mice. Research fields span oncology, hematological malignancies, and traditional medicine.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Rep Med; FBXO3-mediated DUSP9 ubiquitination promotes leukemia stem cell maintenance and tyrosine kinase inhibitor resistance in chronic myeloid leukemia.; Authors - Xudong Li
		J Ethnopharmacol; Xinlikang capsule ameliorates cancer-related fatigue in Lewis lung carcinoma tumor-bearing mice: association with inhibition of oxidative stress and attenuation of JNK activation and bone marrow apoptosis.; Authors - Xingxing Lu