Phospho-MKK7 (Ser271/Thr275) Polyclonal antibody

Phospho-MKK7 (Ser271/Thr275) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29199-1-AP

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MAP2K7 (Ser271/Thr275), PRKMK7

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Validation Data Gallery

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  WB analysis using 29199-1-AP

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 29199-1-AP (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29199-1-AP targets Phospho-MKK7 (Ser271/Thr275) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC038295
• Gene Symbol: MAP2K7
• Gene ID (NCBI): 5609
• RRID: AB_2918246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)