Phospho-P53 (Ser33) Recombinant monoclonal antibody

• 

  WB analysis of T-47D using 87312-1-RR

  Non-treated T-47D cells and etoposide treated T-47D cells were subjected to SDS PAGE followed by western blot with 87312-1-RR (Phospho-P53 (Ser33) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-P53 (Ser33) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87312-1-RR

Clone No.252563C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho P53, Phospho P53 (Ser33), Phospho-P53, Phospho-P53-Ser33, p-p53

---

Tested Applications

• Positive WB detected in: etoposide treated T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87312-1-RR targets Phospho-P53 (Ser33) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: NM_000546
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04637
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. Phosphorylation of p53 at specific amino acid residues plays a crucial role in mediating its various functions, including its role as a transcription factor that regulates the expression of genes involved in the cellular response to stress and DNA damage. Upon severe DNA damage, p53 is phosphorylated at specific amino acid residues, becomes stabilized, and activated to induce apoptosis-related genes. To date, 10 kinases have been identified to phosphorylate p53 for apoptosis at specific serine/threonine residues in the N- and C-terminus domains. (PMID: 30191640)