Phospho-PDH E1 Alpha (Ser293) Polyclonal antibody

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• Phospho-PDH E1 Alpha (Ser293) Polyclonal antibody (29580-1-AP)

Phospho-PDH E1 Alpha (Ser293) Polyclonal Antibody for WB, ELISA

Cat No. 29580-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

p PDH E1 Alpha, p PDH E1 Alpha (Ser293), p PDH E1 Alpha Ser293, Phospho PDH E1 Alpha, Phospho PDH E1 Alpha (Ser293)

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  WB analysis using 29580-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29580-1-AP (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PDH E1 Alpha (18068-1-AP) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29580-1-AP targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• RRID: AB_3669690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A. Multiple post-translational modifications (PTMs) of PDHA1 have been identified, such as acetylation, succinylation, and tyrosine phosphorylation. However, most of these PTMs ultimately affect PDHA1 activity through their influence on the phosphorylation of Ser293, Ser232, and Ser300 which are regulated by PDKs(pyruvate dehydrogenase kinases) and PDPs(pyruvate dehydrogenase phosphatases).(PMID: 34667275)

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Pyruvate metabolism enzyme Dlat induces mitochondria protein hyperacetylation to limit fatty acid oxidation in the HFpEF heart.; Authors - Ying Wang
human	WB	Commun Biol; Crotonylation of ACAT1 K181 contributes to radioresistance by impacting lactate metabolism.; Authors - Shi Chen
human	WB	bioRxiv; Lactylation landscape of mitochondrial proteins in myocardial infarction.; Authors - Ashlesha Kadam
human	WB	iScience; Disturbed mitochondrial maturation in cardiolipin remodeling-deficient cardiomyocytes.; Authors - Nanami Senoo
human	WB	Redox Biol; Lactylation landscape of mitochondrial proteins in myocardial infarction.; Authors - Ashlesha Kadam