Phospho-PDH E1 Alpha (Ser293) Polyclonal antibody

Phospho-PDH E1 Alpha (Ser293) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29580-1-AP

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A

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Validation Data Gallery

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  WB analysis using 29580-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29580-1-AP (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PDH E1 Alpha (18068-1-AP) antibody as a loading control.

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Tested Applications

• Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29580-1-AP targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A. Multiple post-translational modifications (PTMs) of PDHA1 have been identified, such as acetylation, succinylation, and tyrosine phosphorylation. However, most of these PTMs ultimately affect PDHA1 activity through their influence on the phosphorylation of Ser293, Ser232, and Ser300 which are regulated by PDKs(pyruvate dehydrogenase kinases) and PDPs(pyruvate dehydrogenase phosphatases).(PMID: 34667275)