Phospho-PFKFB2 (Ser483) Recombinant monoclonal antibody

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  WB analysis of HeLa using 87525-1-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87525-1-RR (Phospho-PFKFB2 (Ser483) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

• 

  WB analysis of MCF-7 using 87525-1-RR

  Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87525-1-RR (Phospho-PFKFB2 (Ser483) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-PFKFB2 (Ser483) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87525-1-RR

Clone No.252874F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

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Tested Applications

• Positive WB detected in: IGF-1 treated MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87525-1-RR targets Phospho-PFKFB2 (Ser483) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_006212
• Gene Symbol: PFKFB2
• Gene ID (NCBI): 5208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60825
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-PFKFB2 (Ser483) is the active-site-switching modification of the heart-type bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2). Phosphorylation at serine 483, located in the C-terminal regulatory segment, is catalyzed mainly by Akt downstream of PI3K and is essential for maximal 2-kinase activity: it increases the synthesis of fructose-2,6-bisphosphate (F2,6BP), the most potent allosteric activator of 6-phosphofructo-1-kinase (PFK-1), thereby accelerating glycolytic flux and ATP production. (PMID: 34282152)