Phospho-PKN1 (Thr774) Polyclonal antibody, PBS Only

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  WB analysis using 29107-1-AP (same clone as 29107-1-PBS)

  Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29107-1-PBS in a different storage buffer formulation.

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, Indirect ELISA

Cat No. 29107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

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Product Information

29107-1-PBS targets Phospho-PKN1 (Thr774) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase N1
• Calculated Molecular Weight: 942 aa, 104 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC040061
• Gene Symbol: PKN1
• Gene ID (NCBI): 5585
• RRID: AB_2918235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16512
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)