Tested Applications
| Positive WB detected in | IFN gamma and LPS treated THP-1 cells |
| Positive IF/ICC detected in | IFN alpha treated HeLa cells |
| Positive FC (Intra) detected in | IFN gamma treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IF | See 1 publications below |
Product Information
28979-1-AP targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, rabbit, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 1, 91kDa |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 84-91 kDa |
| GenBank Accession Number | BC002704 |
| Gene Symbol | STAT1 |
| Gene ID (NCBI) | 6772 |
| RRID | AB_2935412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-AP | Download protocol |
| WB protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Vet Sci Calycosin inhibits porcine reproductive and respiratory syndrome virus replication and activates RIG-I/IRF3 signaling pathway. | ||
Sci Rep Exploring the anti-inflammatory mechanism of geniposide in rheumatoid arthritis via network pharmacology and experimental validation | ||
Phytother Res Coptisine Improves Liver Inflammation in Sepsis by Regulating STAT1/IRF1/GPX4 Signaling-Mediated Kupffer Cells Ferroptosis | ||
bioRxiv Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice. | ||
Animals (Basel) Ipriflavone Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection via RIG-I/IRF3-Mediated Interferon Signaling. | ||
Invest Ophthalmol Vis Sci Ly93 Inhibits Sphingomyelin Synthesis and Attenuates Inflammation and Injury in Dry Eye Conjunctival Organoids. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Agnieszka (Verified Customer) (02-28-2023) | great working antibody, gives the background
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