Phospho-TBK1 (Ser172) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82382-2-RR (same clone as 82382-2-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82382-2-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82382-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82382-2-RR (same clone as 82382-2-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-TBK1 (Ser172) Recombinant antibody (82382-2-RR, Clone:241787G12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82382-2-PBS in a different storage buffer formulation.

Phospho-TBK1 (Ser172) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82382-2-PBS

Clone No.241787G12

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172

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Published Applications

• WB: See 1 publications below

Product Information

82382-2-PBS targets Phospho-TBK1 (Ser172) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: TANK-binding kinase 1
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC034950
• Gene Symbol: TBK1
• Gene ID (NCBI): 29110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHD2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The serine/threonine protein kinase TBK1, discovered two decades ago as a member of the IκB kinase (IKK) protein kinase family, plays crucial roles in regulating innate immune response by promoting type I interferon (IFN) and modulating NF-κB signaling. The phosphorylation of Ser172 residue on TBK1 is critical and is commonly used as an indicator for its activity. (PMID: 39279883)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Clin Transl Med; Elevation of ISG15 promotes diabetic kidney disease by modulating renal tubular epithelial cell pyroptosis; Authors - Lingzhi Huang