Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal antibody

Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30887-1-AP

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

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Validation Data Gallery

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  WB analysis using 30887-1-AP

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 30887-1-AP (Phospho-p130Cas / BCAR1 (Tyr410) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with p130Cas / BCAR1 antibody (16815-1-AP) subsequently.

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Tested Applications

• Positive WB detected in: pervanadate treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30887-1-AP targets Phospho-p130Cas / BCAR1 (Tyr410) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: breast cancer anti-estrogen resistance 1
• Calculated Molecular Weight: 870 aa, 93 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC062556
• Gene Symbol: BCAR1
• Gene ID (NCBI): 9564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p130Cas, encoded by the breast cancer anti-estrogen resistance 1 (BCAR1) gene, is a scaffold protein that integrates large multi-protein complexes in response to stimuli such as hormones, growth factors, and integrin engagement. Upon environmental signals, Src family members and focal adhesion kinase (FAK) phosphorylate the p130Cas substrate domain (SD). This active p130Cas can recruit adaptor proteins such as Crk and Nck to its phosphorylated SD. (PMID: 34830244)