Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) Polyclonal antibody, PBS Only

• 

  WB analysis using 28735-1-AP (same clone as 28735-1-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28735-1-AP (Phospho-p70(S6K) (Thr389) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28735-1-AP (same clone as 28735-1-PBS)

  Non-treated C6 cells and PMA treated C6 cells were subjected to SDS PAGE followed by western blot with 28735-1-AP (Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28735-1-PBS in a different storage buffer formulation.

Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) Polyclonal Antibody for WB, Indirect ELISA

Cat No. 28735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

p p70(S6K), p p70(S6K) (Thr389), p p70(S6K) Thr389, p70 S6 Kinase (Thr389), p70(S6K) (Thr389)

---

Product Information

28735-1-PBS targets Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Observed Molecular Weight: 65-85 kDa
• GenBank Accession Number: NM_003161
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• RRID: AB_2918197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23443
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)