Phospho-p70(S6K) (Thr389) Polyclonal antibody

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  WB analysis using 28735-1-AP

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28735-1-AP (Phospho-p70(S6K) (Thr389) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-p70(S6K) (Thr389) Polyclonal Antibody for WB, ELISA

Cat No. 28735-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, ELISA

Synonyms

p p70(S6K) Thr389, p p70(S6K) (Thr389), p p70(S6K), p70 S6 Kinase (Thr389), p70(S6K) (Thr389)

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Tested Applications

• Positive WB detected in: IGF-1 treated MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 43 publications below
• IHC: See 2 publications below

Product Information

28735-1-AP targets Phospho-p70(S6K) (Thr389) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Observed Molecular Weight: 65-85 kDa
• GenBank Accession Number: NM_003161
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• RRID: AB_2918197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23443
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70 S6K1 at T389 and p85 S6K1 at T412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

Publications

Species	Application	Title
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Phytomedicine; Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells; Authors - Maolei Xu
human	WB	Oxid Med Cell Longev; Alpha-Ketoglutarate Alleviates Neuronal Apoptosis Induced by Central Insulin Resistance through Inhibiting S6K1 Phosphorylation after Subarachnoid Hemorrhage; Authors - Peng-Fei Ding
human	WB	Mol Oncol; PMID: 29633504; Authors - Yi Xiao
human	WB	Aging (Albany NY); UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.; Authors - Minfen Zhang
human	WB	Int J Mol Sci; Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.; Authors - Juanli Ji