Pikachurin Monoclonal antibody, PBS Only

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  WB analysis of human retina using 66232-1-Ig (same clone as 66232-1-PBS)

  human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66232-1-Ig (same clone as 66232-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 66232-1-Ig (same clone as 66232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Y79 using 66232-1-Ig (same clone as 66232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

Pikachurin Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Cat No. 66232-1-PBS

Clone No.1C6D12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Synonyms

EGFLAM, 1C6D12, Agrin like protein, AGRINL, Agrin-like protein

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Product Information

66232-1-PBS targets Pikachurin in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6362

  Product name: Recombinant human EGFLAM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-152 aa of BC033177

  Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK

• Full Name: EGF-like, fibronectin type III and laminin G domains
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 111 kDa, 55-60 kDa
• GenBank Accession Number: BC033177
• Gene Symbol: Pikachurin
• Gene ID (NCBI): 133584
• RRID: AB_2881623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q63HQ2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).