Plakophilin 2 Polyclonal antibody

Plakophilin 2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26479-1-AP

Synonyms

ARVD9, PKP2, plakophilin 2

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Validation Data Gallery

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  WB analysis using 26479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26479-1-AP

  IP result of anti-Plakophilin 2 (IP:26479-1-AP, 4ug; Detection:26479-1-AP 1:1000) with HepG2 cells lysate 2200 ug.

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Tested Applications

• Positive WB detected in: Caco-2 cells, HepG2 cells, rat heart tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26479-1-AP targets Plakophilin 2 in WB, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Plakophilin 2 fusion protein Ag24155
• Full Name: plakophilin 2
• Calculated Molecular Weight: 837 aa, 93 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC094762
• Gene Symbol: PKP2
• Gene ID (NCBI): 5318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakophilin 2 also known as PKP2, belongs to the beta-catenin family. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10828611). Mutations in PKP2 are associated with Arrhythmogenic right ventricular dysplasia, familial, 9 (ARVD9)(PMID: 15489853).