Protein A Monoclonal antibody

Protein A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

staphylococcus aureus and More (3)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G6A6

Cat no : 66945-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66945-1-Ig

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ProteinA using 66945-1-Ig

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

66945-1-Ig targets Protein A in WB, IP, ELISA applications and shows reactivity with staphylococcus aureus samples.

• Tested Reactivity: staphylococcus aureus
• Cited Reactivity: human, mouse, yeast
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Protein A
• GenBank Accession Number: EU695225
• RRID: AB_2882269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publications

Species	Application	Title
	IP	Virus Res; HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation.; Authors - Qian Wang
human		J Biochem; Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry.; Authors - Katsutoshi Yoshizato
mouse	WB	Hepatology; Concerted synergy between viral-specific IgG and CD8 + T cells is critical for clearance of an HCV-related rodent hepacivirus; Authors - John Gridley
yeast	WB	Proc Natl Acad Sci U S A; DNA polymerase delta governs parental histone transfer to DNA replication lagging strand; Authors - Congcong Tian