Validation Data Gallery
|Positive WB detected in||mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11947-1-AP targets RAB5A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB5A fusion protein Ag2549|
|Full Name||RAB5A, member RAS oncogene family|
|Calculated molecular weight||215 aa, 24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC001267|
|Gene ID (NCBI)||5868|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Identification of TMEM230 mutations in familial Parkinson's disease.
Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.
RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.
Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.
Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.
Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sophy (Verified Customer) (02-27-2023)
The antibody was used at 1:1000 for western blot. The signal showed up but not as strong as expected. But since this was the only antibody purchased for Rab5A, I had no comparison to antibodies to other vendors.
Xin (Verified Customer) (01-24-2022)
Very good antibody in WB (25 kD) with a high titer
Tom (Verified Customer) (11-13-2020)
HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.
Aamir (Verified Customer) (01-19-2020)
Used in HEK cells. Good signal for WB and IF