• Featured Product
  • KD/KO Validated

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11947-1-AP

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, human brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11947-1-AP targets RAB5A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB5A fusion protein Ag2549
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB5A antibody 11947-1-APDownload protocol
IHC protocol for RAB5A antibody 11947-1-APDownload protocol
IP protocol for RAB5A antibody 11947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Development

A crucial role for Arf6 in the response of commissural axons to Slit.

Authors - Mariko Kinoshita-Kawada
  • KD Validated
mouseWB

FASEB J

Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.

Authors - Kobina Essandoh
humanWB,IHC

Mol Med Rep

Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling.

Authors - Di Zhang
humanWB, IHC

Int J Clin Exp Pathol

MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A.

Authors - Yuanqing Pan
humanWB,IHC

Virchows Arch

Expression of Rab5a correlates with tumor progression in pancreatic carcinoma.

Authors - Yuandong Li
  • KD Validated
humanWB,IF

Am J Transl Res

Rab5a suppresses autophagy to promote drug resistance in cancer cells.

Authors - Wenxia Xu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (11-13-2020)

HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB5A Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11947-1-AP)
FH

Aamir (Verified Customer) (01-19-2020)

Used in HEK cells. Good signal for WB and IF

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: HEKs