|Positive WB detected in||mouse brain tissue, rat brain tissue, human brain tissue, HeLa cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11947-1-AP targets RAB5A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB5A fusion protein Ag2549|
|Full Name||RAB5A, member RAS oncogene family|
|Calculated molecular weight||215 aa, 24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC001267|
|Gene ID (NCBI)||5868|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
A crucial role for Arf6 in the response of commissural axons to Slit.
Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.
Mol Med Rep
Rab5a is overexpressed in oral cancer and promotes invasion through ERK/MMP signaling.
Int J Clin Exp Pathol
MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A.
Expression of Rab5a correlates with tumor progression in pancreatic carcinoma.
Am J Transl Res
Rab5a suppresses autophagy to promote drug resistance in cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (11-13-2020)
HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.
Aamir (Verified Customer) (01-19-2020)
Used in HEK cells. Good signal for WB and IF