• Featured Product
  • KD/KO Validated

RAB5A Polyclonal antibody

RAB5A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11947-1-AP

Synonyms

Cell and organelle markers, Endosome Marker, RAB5, RAB5A, Ras related protein Rab 5A



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11947-1-AP targets RAB5A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB5A fusion protein Ag2549
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB5A antibody 11947-1-APDownload protocol
IHC protocol for RAB5A antibody 11947-1-APDownload protocol
IP protocol for RAB5A antibody 11947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
humanWB,IP

Autophagy

Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

Authors - Amy H Ponsford
mouseWB

Autophagy

RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

Authors - Xin Jin
mouseWB,IHC

Diabetes

Atorvastatin Targets the Islet Mevalonate Pathway to Dysregulate mTOR Signaling and Reduce β-Cell Functional Mass.

Authors - Linyan Shen
  • KD Validated
ratWB, IF

Sci Signal

Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.

Authors - Kong-Yan Wu
humanWB,IF,IP

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xin (Verified Customer) (01-24-2022)

Very good antibody in WB (25 kD) with a high titer

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: mouse brain lysate
FH

Tom (Verified Customer) (11-13-2020)

HEK293T cell extracts (10ug/lane). Primary antibody (1:2000) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-rabbit HRP secondary antibody (1:10,000) incubated for 1 hour at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB5A Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11947-1-AP)
FH

Aamir (Verified Customer) (01-19-2020)

Used in HEK cells. Good signal for WB and IF

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: HEKs