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Rac1 Antibody

Rac1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, coIP, ELISA

Conjugate

Unconjugated

Cat no : 24072-1-AP

Synonyms

MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 24072-1-AP immunogen is Fusion Protein Rac1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity HUMAN, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rac1 fusion protein Ag17779
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession number BC004247
Gene symbol RAC1
Gene ID (NCBI) 5879
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Protocols

Product Specific Protocols
WB protocol for Rac1 antibody 24072-1-APDownload protocol
IF protocol for Rac1 antibody 24072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Pharmacol Biochem Behav

Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice.

Authors - Yuchan Wang
ratWB

Int J Mol Med

PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R.

Authors - Qixiang Zhao
human,mouseIHC,WB

Cancer Res

CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder.

Authors - Xin Chen
humanWB

Oncotarget

Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.

Authors - Yilong Li
humanWB

Cells

Modulation of the Pol II CTD Phosphorylation Code by Rac1 and Cdc42 Small GTPases in Cultured Human Cancer Cells and Its Implication for Developing a Synthetic-Lethal Cancer Therapy.

Authors - Bo Zhang
IF

Mol Med Rep

NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats.

Authors - Hongwei Kan