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Rac1 Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 24072-1-AP

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Con: 45 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, WB
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Images See all

24072-1-AP;human brain tissue

human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP(RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

24072-1-AP;HeLa cells

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 24072-1-AP(Rac1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

24072-1-AP;human colon tissue

Immunohistochemistry of paraffin-embedded human colon tissue slide using 24072-1-AP( RAC1 Antibody) at dilution of 1:100 (under 10x lens).

24072-1-AP;human colon tissue

Immunohistochemistry of paraffin-embedded human colon tissue slide using 24072-1-AP( RAC1 Antibody) at dilution of 1:100 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in:
human colon tissue, human colon tissue, human small intestine tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Rac1 fusion protein ag17779
Full name:
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC004247
Gene ID (NCBI):
5879
Gene symbol
RAC1
Synonyms
MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25
Background

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.


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