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- KD/KO Validated
Rac1 Antibody
Rac1 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, coIP, ELISA
Conjugate
Unconjugated
Cat no : 24072-1-AP
Synonyms
MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 21 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
The 24072-1-AP immunogen is Fusion Protein Rac1 protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | HUMAN, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Rac1 fusion protein Ag17779 |
| Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC004247 |
| Gene symbol | RAC1 |
| Gene ID (NCBI) | 5879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Rac1 antibody 24072-1-AP | Download protocol |
| IF protocol for Rac1 antibody 24072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmacol Biochem Behav Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice. | ||
Int J Mol Med PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R. | ||
Cancer Res CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder. | ||
Oncotarget Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network. | ||
Cells Modulation of the Pol II CTD Phosphorylation Code by Rac1 and Cdc42 Small GTPases in Cultured Human Cancer Cells and Its Implication for Developing a Synthetic-Lethal Cancer Therapy. | ||
Mol Med Rep NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats. |
