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Rac1 Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 24072-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Rac1 Antibody WB mouse brain tissue 24072-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 24072-1-AP (Rac1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Rac1 Antibody IHC human colon tissue 24072-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24072-1-AP( RAC1 Antibody) at dilution of 1:100 (under 10x lens).

Rac1 Antibody IHC human colon tissue 24072-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24072-1-AP( RAC1 Antibody) at dilution of 1:100 (under 40x lens).

Rac1 Antibody IF HeLa cells 24072-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 24072-1-AP (Rac1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected in:
human colon tissue, human small intestine tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Rac1 fusion protein Ag17779
Full name:
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC004247
Gene ID (NCBI):
5879
Gene symbol
RAC1
Synonyms
MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

Background

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.


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