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- KD/KO Validated
Rac1 Polyclonal antibody
Rac1 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, coIP, ELISA
Conjugate
Unconjugated
Cat no : 24072-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 50 publications below |
IHC | See 6 publications below |
IF | See 6 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 24072-1-AP is Rac1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Rac1 fusion protein Ag17779 |
Full Name | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC004247 |
Gene symbol | RAC1 |
Gene ID (NCBI) | 5879 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Protocols
Product Specific Protocols | |
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WB protocol for Rac1 antibody 24072-1-AP | Download protocol |
IF protocol for Rac1 antibody 24072-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Biol Sci Spheroid Formation Enhances the Regenerative Capacity of Nucleus Pulposus Cells via Regulating N-CDH and ITGβ1 Interaction | ||
Cell Death Dis Tumour-derived exosomal lncRNA-SOX2OT promotes bone metastasis of non-small cell lung cancer by targeting the miRNA-194-5p/RAC1 signalling axis in osteoclasts. | ||
Cell Death Dis Overexpression of DTL enhances cell motility and promotes tumor metastasis in cervical adenocarcinoma by inducing RAC1-JNK-FOXO1 axis. | ||
Pharmacol Biochem Behav Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice. | ||
Int J Mol Med PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R. | ||
Cancer Res CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder. |