RAD51AP1 Monoclonal antibody, PBS Only

• 

  WB analysis using 68560-1-Ig (same clone as 68560-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68560-1-PBS in a different storage buffer formulation.

RAD51AP1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68560-1-PBS

Clone No.2D8B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

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Product Information

68560-1-PBS targets RAD51AP1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28385

  Product name: Recombinant human RAD51AP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-352 aa of BC016330

  Sequence: MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELKQDKPKPNLNNLRKEEIPVQEKTPKKRLPEGTFSIPASAVPCTKMALDDKLYQRDLEVALALSVKELPTVTTNVQNSQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAAAQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKKEVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIRSPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATST

• Full Name: RAD51 associated protein 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC016330
• Gene Symbol: RAD51AP1
• Gene ID (NCBI): 10635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96B01
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].