RBBP6 Recombinant antibody, PBS Only

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  WB analysis using 85884-5-RR (same clone as 85884-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85884-5-RR (RBBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85884-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85884-5-RR (same clone as 85884-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RBBP6 antibody (85884-5-RR, Clone: 250142C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85884-5-PBS in a different storage buffer formulation.

RBBP6 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85884-5-PBS

Clone No.250142C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

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Product Information

85884-5-PBS targets RBBP6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBBP6 fusion protein Ag2484
• Full Name: retinoblastoma binding protein 6
• Calculated Molecular Weight: 1792 aa, 200 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC029352
• Gene Symbol: RBBP6
• Gene ID (NCBI): 5930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6E9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].