Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10196-1-AP targets RBM14 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0254 Product name: Recombinant human RBM14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 465-666 aa of BC000488 Sequence: PVVQTQLNSYGAQASMGLSGSYGAQSAAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLSMSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTKSSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQ Predict reactive species |
| Full Name | RNA binding motif protein 14 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC000488 |
| Gene Symbol | RBM14 |
| Gene ID (NCBI) | 10432 |
| RRID | AB_2175748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBM14 is a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBM14 antibody 10196-1-AP | Download protocol |
| WB protocol for RBM14 antibody 10196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RBM14 Polyclonal antibody (10196-1-AP) has 8 citations published in journals including Nature Structural & Molecular Biology, Cell Death & Disease, Experimental Molecular Medicine, Journal of Translational Medicine, International Journal of Biological Macromolecules, Journal of Virology, Brain Research, and Translational Cancer Research. Associated research fields span triple-negative breast cancer, colorectal cancer liver metastasis, ALS, Parkinson's disease, viral infections (Senecavirus A and porcine epidemic diarrhea virus), protein-protein interaction networks, and osteosarcoma.
| Species | Application | Title |
|---|---|---|
Exp Mol Med The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability | ||
Cell Death Dis OTUD6B-mediated K48 deubiquitination of FXR1 forms a positive feedback loop activating MEK2/ERK signaling in colorectal cancer liver metastasis. | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
J Transl Med A diagnostic model for Parkinson's disease based on circadian rhythm-related genes | ||
Int J Biol Macromol Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection | ||
J Virol RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway |
