RBM15 Monoclonal antibody

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  WB analysis using 66059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 66059-1-Ig

  WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

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  WB analysis of human brain using 66059-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66059-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66059-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66059-1-Ig

  IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66059-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

RBM15 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 66059-1-Ig

Clone No.4A1A4

Publications(6)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, HeLa cells, human heart tissue, HEK-293 cells, HEK293 cells, MCF-7 cells, LNCaP cells, Jurkat cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

66059-1-Ig targets RBM15 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM15 fusion protein Ag17970
• Full Name: RNA binding motif protein 15
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC006397
• Gene Symbol: RBM15
• Gene ID (NCBI): 64783
• RRID: AB_11042326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96T37
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
human	WB	Elife; Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.; Authors - Li Zhang
	WB	EMBO Rep; The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.; Authors - Ngoc-Tung Tran
human	IHC	J Gene Med; The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.; Authors - Yujie Li
human	WB	Exp Mol Med; The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism; Authors - Su Hwan Park ; KD Validated
human	RIP,IP,IF	Oncogene; RBM15 facilitates osimertinib resistance of lung adenocarcinoma through m6A-dependent epigenetic silencing of SPOCK1; Authors - Hongxiang Li