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RBM15 Monoclonal antibody
RBM15 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66059-1-Ig
Validation Data Gallery
|Positive WB detected in
|A549 cells, human brain tissue, HeLa cells, human heart tissue, HEK-293 cells, HEK293 cells, MCF-7 cells, LNCaP cells, Jurkat cells, K-562 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:200-1:800
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66059-1-Ig targets RBM15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2a
|RBM15 fusion protein Ag17970
|RNA binding motif protein 15
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
|Product Specific Protocols
|WB protocol for RBM15 antibody 66059-1-Ig
|IHC protocol for RBM15 antibody 66059-1-Ig
|IF protocol for RBM15 antibody 66059-1-Ig
|IP protocol for RBM15 antibody 66059-1-Ig
|Click here to view our Standard Protocols
Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.
Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.
J Gene Med
The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.