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RBM15 Monoclonal antibody

RBM15 Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4A1A4

Cat no : 66059-1-Ig

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN



Tested Applications

Positive WB detected inHela cells, HEK293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 66059-1-Ig is RBM15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBM15 fusion protein Ag17970
Full Name RNA binding motif protein 15
Calculated molecular weight 105 kDa
Observed molecular weight 107 kDa
GenBank accession numberBC006397
Gene symbol RBM15
Gene ID (NCBI) 64783
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
WB protocol for RBM15 antibody 66059-1-IgDownload protocol
IHC protocol for RBM15 antibody 66059-1-IgDownload protocol
IP protocol for RBM15 antibody 66059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

EMBO Rep

The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.

Authors - Ngoc-Tung Tran
humanWB

Elife

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Authors - Li Zhang