RBM15 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66059-1-Ig (same clone as 66059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 66059-1-Ig (same clone as 66059-1-PBS)

  WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66059-1-Ig (same clone as 66059-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66059-1-Ig (same clone as 66059-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

  IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51442-1

  Cytometric bead array sample test of MP51442-1, RBM15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66059-1-PBS. Detection antibody: 66059-2-PBS.

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  Cytometric bead array standard curve of MP51442-1

  Cytometric bead array standard curve of MP51442-1, RBM15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66059-1-PBS. Detection antibody: 66059-2-PBS. Standard:Ag17970. Range: 0.391-100 ng/mL.

• Featured Product
• KD/KO Validated

RBM15 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Cat No. 66059-1-PBS

Clone No.4A1A4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Synonyms

4A1A4, One-twenty two protein 1, OTT, OTT1, RBM 15

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Product Information

66059-1-PBS targets RBM15 as part of a matched antibody pair:

MP51442-1: 66059-1-PBS capture and 66059-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17970

  Product name: Recombinant human RBM15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 658-957 aa of BC006397

  Sequence: SSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL

• Full Name: RNA binding motif protein 15
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC006397
• Gene Symbol: RBM15
• Gene ID (NCBI): 64783
• RRID: AB_11042326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96T37
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.