|Positive WB detected in||HeLa cells, A375 cells, MCF-7 cells, Raji cells, HepG2 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human testis tissue, human lung tissue, human brain tissue, human kidney tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14363-1-AP targets RBM3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, squirrel|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBM3 fusion protein Ag5934|
|Full Name||RNA binding motif (RNP1, RRM) protein 3|
|Calculated molecular weight||17 kDa|
|Observed molecular weight||17-20 kDa|
|GenBank accession number||BC006825|
|Gene ID (NCBI)||5935|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).
|Product Specific Protocols|
|WB protocol for RBM3 antibody 14363-1-AP||Download protocol|
|IHC protocol for RBM3 antibody 14363-1-AP||Download protocol|
|IF protocol for RBM3 antibody 14363-1-AP||Download protocol|
|IP protocol for RBM3 antibody 14363-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Oncol
RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression.
The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.
Front Cell Dev Biol
The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.
Neuroprotection via RNA-binding protein RBM3 expression is regulated by hypothermia but not by hypoxia in human SK-N-SH neurons.
Exp Mol Pathol
Role of RBM3 in the regulation of cell proliferation in hepatocellular carcinoma.
Life Sci Alliance
TrkB signaling regulates the cold-shock protein RBM3-mediated neuroprotection.