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RBM3 Polyclonal antibody

RBM3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14363-1-AP

Synonyms

IS1 RNPL, Putative RNA binding protein 3, RBM3, RNA binding motif protein 3, RNPL



Tested Applications

Positive WB detected inHeLa cells, A375 cells, MCF-7 cells, Raji cells, HepG2 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue, human lung tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14363-1-AP targets RBM3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM3 fusion protein Ag5934
Full Name RNA binding motif (RNP1, RRM) protein 3
Calculated molecular weight 17 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC006825
Gene symbol RBM3
Gene ID (NCBI) 5935
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).

Protocols

Product Specific Protocols
WB protocol for RBM3 antibody 14363-1-APDownload protocol
IHC protocol for RBM3 antibody 14363-1-APDownload protocol
IF protocol for RBM3 antibody 14363-1-APDownload protocol
IP protocol for RBM3 antibody 14363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression.

Authors - Ping Chen

EBioMedicine

The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.

Authors - Wei Dong
mouseWB

Front Cell Dev Biol

The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.

Authors - Jingyi Yan
humanWB

Hypoxia (Auckl)

Neuroprotection via RNA-binding protein RBM3 expression is regulated by hypothermia but not by hypoxia in human SK-N-SH neurons.

Authors - Lisa-Maria Rosenthal
humanWB,IHC

Exp Mol Pathol

Role of RBM3 in the regulation of cell proliferation in hepatocellular carcinoma.

Authors - Xiaobing Miao
mouseWB

Life Sci Alliance

TrkB signaling regulates the cold-shock protein RBM3-mediated neuroprotection.

Authors - Diego Peretti