RBM4 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

  WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60292-1-Ig (same clone as 60292-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60292-1-PBS

Clone No.3H3F12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4

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Product Information

60292-1-PBS targets RBM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2190

  Product name: Recombinant human RBM4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-177 aa of BC021120

  Sequence: MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGEPPSLGRGLNTRLCAENGWISKRRGLVKITAVGWLVMKK

• Full Name: RNA binding motif protein 4
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC021120
• Gene Symbol: RBM4
• Gene ID (NCBI): 5936
• RRID: AB_2881407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BWF3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.