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- KD/KO Validated
RBPJ Polyclonal antibody
RBPJ Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IHC, ELISA
Cat no : 14613-1-AP
Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, HEK-293 cells, HeLa cells, HepG2 cells, human heart tissue, human liver tissue, MCF-7 cells, mouse pancreas tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 8 publications below|
|IHC||See 3 publications below|
14613-1-AP targets RBPJ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RBPJ fusion protein Ag6206|
|Full Name||recombination signal binding protein for immunoglobulin kappa J region|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC064976|
|Gene ID (NCBI)||3516|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.
|Product Specific Protocols|
|WB protocol for RBPJ antibody 14613-1-AP||Download protocol|
|IHC protocol for RBPJ antibody 14613-1-AP||Download protocol|
|IP protocol for RBPJ antibody 14613-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis.
J Biol Chem
RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal.
DAPT Attenuates Cadmium-Induced Toxicity in Mice by Inhibiting Inflammation and the Notch/HES-1 Signaling Axis.
Salidroside alleviates cadmium-induced toxicity in mice by restoring the notch/HES-1 and RIP1-driven inflammatory signaling axis.
Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation
Edaravone attenuates cadmium-induced toxicity by inhibiting oxidative stress and inflammation in ICR mice.