RC3H1 Polyclonal antibody

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  WB analysis using 31234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 31234-1-AP

  WB result of RC3H1 antibody (31234-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RC3H1 transfected HEK-293T cells.

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  IHC staining of mouse pancreas using 31234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 31234-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 31234-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31234-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA2025, RING finger protein 198, RNF198, ROQUIN

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Tested Applications

• Positive WB detected in: 4T1 cells, HEK-293T cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31234-1-AP targets RC3H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35089

  Product name: Recombinant human RC3H1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1000-1133 aa of NM_001300850

  Sequence: NTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP

• Full Name: ring finger and CCCH-type zinc finger domains 1
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_001300850
• Gene Symbol: RC3H1
• Gene ID (NCBI): 149041
• RRID: AB_3669912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5TC82
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)