Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17008-1-AP targets RECQL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10707 Product name: Recombinant human RECQL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 297-652 aa of BC013277 Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR Predict reactive species |
| Full Name | RecQ protein-like 4 |
| Calculated Molecular Weight | 1208 aa, 133 kDa |
| Observed Molecular Weight | 145-150 kDa |
| GenBank Accession Number | BC013277 |
| Gene Symbol | RECQL4 |
| Gene ID (NCBI) | 9401 |
| RRID | AB_2238324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94761 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| IHC protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| IP protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| WB protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RECQL4 Polyclonal antibody (Cat# 17008-1-AP) has 10 citations. Journals include Nature Communications, Cancer Biology & Medicine, Scientific Reports, Translational Oncology, FASEB Journal, Drug Development Research, Journal of Biological Chemistry, Journal of Neuropathology & Experimental Neurology, and bioRxiv. Research fields span DNA replication and damage response, DNA repair, redox homeostasis, and cancer biology (esophageal, cervical, breast, glioma, and nerve sheath tumors), with applications in WB, IP, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Nat Commun Selective interactions at pre-replication complexes categorize baseline and dormant origins | ||
Cancer Biol Med RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.
| ||
Transl Oncol Unraveling the mechanisms of RECQL4-mediated cervical cancer progression through the PI3K/AKT pathway | ||
Sci Rep PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner. | ||
FASEB J UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair. |
