REDD1 Monoclonal antibody, PBS Only

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  WB analysis using 67059-1-Ig (same clone as 67059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67059-1-Ig (same clone as 67059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

REDD1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67059-1-PBS

Clone No.3A2C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

DDIT4, 3A2C10, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, REDD-1

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Product Information

67059-1-PBS targets REDD1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0965

  Product name: Recombinant human REDD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-232 aa of BC007714

  Sequence: MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC

• Full Name: DNA-damage-inducible transcript 4
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC007714
• Gene Symbol: REDD1/DDIT4
• Gene ID (NCBI): 54541
• RRID: AB_2882369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NX09
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].