ChromoTek rat anti RFP Monoclonal antibody (5F8)

Rat monoclonal [5F8] to Red Fluorescent Proteins (RFP)

Host/IsoType

Rat/IgG2a

Specificity

RFP and RFP derivates

Applications

IF, ELISA

Conjugate

Unconjugated

Clone No.

5F8

Cat no : 5f8

Synonyms

RFP, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

Rat monoclonal [5F8] to Red Fluorescent Proteins (RFP)

DescriptionRFP rat monoclonal [5F8] for IF, ELISA
ApplicationsIF, ELISA
Specificity/TargetmOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, mPlum
For the complete list, please click here: Fluorescent protein specificity table antibodies
Recommended DilutionImmunofluorescence: 1:1,000
Host/IsoTypeRat/IgG2a
ClassMonoclonal
TypeAntibody
ImmunogenRed fluorescent protein (RFP)
UniProtKBQ9U6Y8
Clone No.5F8
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A or G purified
RRIDAB_2336064
Storage BufferPBS, 50% glycerol, preservative: 0.09% sodium azide.
Storage ConditionShipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.
BackgroundRed fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.
The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).
When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a rat monoclonal antibody raised against RFP, reactive to a variety of RFP derivates, including mOrange, DsRed, mCherry, mRFP, mRFPRuby, mScarlet, tdTomato, mKate2, and mPlum.

Publications

ApplicationTitle
IF

Nature

Fast and sensitive GCaMP calcium indicators for imaging neural populations

Authors - Yan Zhang
WB

Nature

Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios.

Authors - Philipp S Hoppe
IHC

Nature

Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.

Authors - Joon-Hyuk Lee
IF

Nature

Equalizing excitation-inhibition ratios across visual cortical neurons.

Authors - Mingshan Xue
IF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
IHC

Nature

Temporal controls over inter-areal cortical projection neuron fate diversity.

Authors - Esther Klingler

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Prabha (Verified Customer) (09-11-2023)

The RFP antibody 5F8 is really excellent. Immunodetection shows specific detection without any background signal.

  • Applications: Western Blot,Immunoprecipitation,Protein purification
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Plant protein extracts
FH

Ramzi (Verified Customer) (01-24-2023)

I have tested the anti 5f8 Rat and the secondary I used anti-GFP Rat 488. I used vibratome brain sections from creER2 tomato DsRed mice. I tested in 2 different ratio 1:200 and 1:1000 both worked very will and this is the images I took.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200, 1:1000
  • Cell Tissue Type: Brain Tissue