RFX1 Monoclonal antibody, PBS Only

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  WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68057-1-Ig (same clone as 68057-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68057-1-Ig (same clone as 68057-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 68057-1-Ig (same clone as 68057-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

RFX1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68057-1-PBS

Clone No.1G10H6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Synonyms

RFX, EF-C, EF C, 1G10H6

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Product Information

68057-1-PBS targets RFX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RFX1 fusion protein Ag25442
• Full Name: regulatory factor X, 1 (influences HLA class II expression)
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC049826
• Gene Symbol: RFX1
• Gene ID (NCBI): 5989
• RRID: AB_2918798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P22670
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).