RFXAP Polyclonal antibody

RFXAP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20188-1-AP

Synonyms

RFX associated protein, RFXAP

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Validation Data Gallery

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  WB analysis of HeLa using 20188-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP (RFXAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20188-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP (RFXAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 20188-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20188-1-AP (RFXAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20188-1-AP is RFXAP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFXAP fusion protein Ag14100
• Full Name: regulatory factor X-associated protein
• Calculated Molecular Weight: 272 aa, 28 kDa
• Observed Molecular Weight: 23-26 kDa
• GenBank Accession Number: BC026088
• Gene Symbol: RFXAP
• Gene ID (NCBI): 5994
• RRID: AB_10697835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RFXAP is a subunit of the RFX complex that binds to the X-box of MHC II promoters. It's involved in Bare lymphocyte syndrome 2 (BLS2).