|Positive WB detected in||HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27248-1-AP is RICTOR Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RICTOR fusion protein Ag25649|
|Full Name||rapamycin-insensitive companion of mTOR|
|Calculated molecular weight||192 kDa|
|Observed molecular weight||192 kDa|
|GenBank accession number||BC029608|
|Gene ID (NCBI)||253260|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.
J Cell Physiol
Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy.
J Genet Genomics
mTOR-S6K1 pathway mediates cytoophidium assembly.
J Sex Med
Rapamycin Supplementation May Ameliorate Erectile Function in Rats With Streptozotocin-Induced Type 1 Diabetes by Inducing Autophagy and Inhibiting Apoptosis, Endothelial Dysfunction, and Corporal Fibrosis.
Exp Cell Res
TXNIP deficiency mitigates podocyte apoptosis via restraining the activation of mTOR or p38 MAPK signaling in diabetic nephropathy.
Am J Physiol Heart Circ Physiol
Galectin-3 Mediates Cardiac Remodeling Caused by Impaired Glucose and Lipid Metabolism Through Inhibiting Two Pathways of Activating Akt.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
The antibody recognizes RICTOR which is around ~200kD. It is the top most band. The first two lanes were cell lysates and show that the antibody recognizes other proteins non-specifically. The last lane contained eluted proteins that are presumably proximal to the cell membrane. RICTOR shows up there cleanly.