Tested Applications
| Positive WB detected in | Jurkat cells, RAW 264.7 cells, HSC-T6 cells, Ramos cells, K-562 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66647-1-Ig targets RNF126 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13816 Product name: Recombinant human RNF126 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-326 aa of BC001442 Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL Predict reactive species |
| Full Name | ring finger protein 126 |
| Calculated Molecular Weight | 326 aa, 36 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC001442 |
| Gene Symbol | RNF126 |
| Gene ID (NCBI) | 55658 |
| RRID | AB_2858189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BV68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RNF126 antibody 66647-1-Ig | Download protocol |
| WB protocol for RNF126 antibody 66647-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNF126 Antibody (Cat# 66647-1-Ig) has 14 total citations published in journals including Molecular Cell, Journal of Clinical Investigation, Advanced Science, Cell Death & Disease, ACS Central Science, Cell Chemical Biology, European Journal of Medicinal Chemistry, International Journal of Molecular Sciences, Cell Signaling, Acta Biochimica et Biophysica Sinica, Journal of Cellular Physiology, Experimental Eye Research, and bioRxiv. Research fields span ubiquitin-mediated protein degradation, proteasomal quality control, cancer biology (gastric, ovarian, pancreatic), ferroptosis, cellular senescence, viral pathogenesis, and molecular glue degrader design.
| Species | Application | Title |
|---|---|---|
Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.
| ||
J Clin Invest Poly(ADP-ribose) glycohydrolase enforces p21 degradation via dePARylation to promote gastric cancer progression | ||
Adv Sci (Weinh) Dipyridamole Acts as Clinical Ferroptosis Inhibitor to Prevent from Tissue Injury | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination. | ||
ACS Cent Sci DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders
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