RNF126 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66647-1-Ig (same clone as 66647-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66647-1-Ig (same clone as 66647-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Monoclonal antibody (66647-1-Ig, Clone:1E1B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RNF126 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 66647-1-PBS

Clone No.1E1B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

1E1B10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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Product Information

66647-1-PBS targets RNF126 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13816

  Product name: Recombinant human RNF126 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-326 aa of BC001442

  Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL

• Full Name: ring finger protein 126
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC001442
• Gene Symbol: RNF126
• Gene ID (NCBI): 55658
• RRID: AB_2858189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV68
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).