RNF167 Polyclonal antibody, PBS Only

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  WB analysis of SH-SY5Y using 24618-1-AP (same clone as 24618-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24618-1-AP (same clone as 24618-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 24618-1-AP (same clone as 24618-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24618-1-AP (same clone as 24618-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 24618-1-AP (same clone as 24618-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RNF167 antibody (24618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24618-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RNF167 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 24618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105

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Product Information

24618-1-PBS targets RNF167 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18383

  Product name: Recombinant human RNF167 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-200 aa of BC010139

  Sequence: IRATSDHNASMDFADLPALFGATLSQEGLQGFLVEAHPDNACSPIAPPPPAPVNGSVFIALLRRFDCNFDLKVLNAQKAGYGAAVVHNVNSNELLNMVWNSEEIQQQIWIPSVFIGERSSEYLRALFVYEKGARVLLVPDNTFPLGYYLIPFTGIVGLLVLAMGAVMIARCIQHR

• Full Name: ring finger protein 167
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC010139
• Gene Symbol: RNF167
• Gene ID (NCBI): 26001
• RRID: AB_2879642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H6Y7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).