RNF167 Polyclonal antibody

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  WB analysis of SH-SY5Y using 24618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 24618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24618-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HaCaT using 24618-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RNF167 antibody (24618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

RNF167 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24618-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, mouse brain tissue, mouse testis tissue, SH-SY5Y cells, HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

24618-1-AP targets RNF167 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18383

  Product name: Recombinant human RNF167 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-200 aa of BC010139

  Sequence: IRATSDHNASMDFADLPALFGATLSQEGLQGFLVEAHPDNACSPIAPPPPAPVNGSVFIALLRRFDCNFDLKVLNAQKAGYGAAVVHNVNSNELLNMVWNSEEIQQQIWIPSVFIGERSSEYLRALFVYEKGARVLLVPDNTFPLGYYLIPFTGIVGLLVLAMGAVMIARCIQHR

• Full Name: ring finger protein 167
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC010139
• Gene Symbol: RNF167
• Gene ID (NCBI): 26001
• RRID: AB_2879642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H6Y7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.; Authors - Tingting Li ; KD Validated
human	WB	FASEB J; RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation; Authors - Zhenzhen Yan
mouse,human	WB,IP	Nat Commun; RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways; Authors - Miao He ; KD Validated