RNF168 Polyclonal antibody

• 

  WB analysis of HepG2 using 21393-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 21393-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

• 

  WB analysis of Jurkat using 21393-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21393-1-AP

  IP result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IF Staining of HepG2 using 21393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF168 antibody (21393-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RNF168 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 21393-1-AP

Publications(14)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

RING-type E3 ubiquitin transferase RNF168, RING finger protein 168, hRNF168, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF168

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human brain tissue, Jurkat cells, Transfected HEK-293 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

21393-1-AP targets RNF168 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16055

  Product name: Recombinant human RNF168 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 221-571 aa of BC033791

  Sequence: NTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK

• Full Name: ring finger protein 168
• Calculated Molecular Weight: 571 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC033791
• Gene Symbol: RNF168
• Gene ID (NCBI): 165918
• RRID: AB_10733883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB,IHC,IF	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
human	IP	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB,IF	J Cell Biol; DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response; Authors - Tessa Arends

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band, no background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)

FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band without background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)