RNF6 Polyclonal antibody, PBS Only

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  WB analysis using 20437-1-AP (same clone as 20437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

  IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 20437-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

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Product Information

20437-1-PBS targets RNF6 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14209

  Product name: Recombinant human RNF6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC034688

  Sequence: MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRTNPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSSNIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHVWENGARSNVTVRNTNQRLEP

• Full Name: ring finger protein (C3H2C3 type) 6
• Calculated Molecular Weight: 685 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC034688
• Gene Symbol: RNF6
• Gene ID (NCBI): 6049
• RRID: AB_10666853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y252
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].