RON/MST1R Monoclonal antibody, PBS Only

• 

  WB analysis of BxPC-3 using 67954-1-Ig (same clone as 67954-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67954-1-Ig (same clone as 67954-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67954-1-Ig (same clone as 67954-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67954-1-Ig (same clone as 67954-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.

RON/MST1R Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67954-1-PBS

Clone No.2D8D1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MST1R, 2D8D1, CD136, CDw136, EC:2.7.10.1

---

Product Information

67954-1-PBS targets RON/MST1R in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28289

  Product name: Recombinant human RON, MST1R protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1038-1332 aa of BQ936677 NM_002447

  Sequence: ASFSDSEDESCVPLLRKESIQLRDLDSALLAEVKDVLIPHERVVTHSDRVIGKGHFGVVYHGEYIDQAQNRIQCAIKSLSRITEMQQVEAFLREGLLMRGLNHPNVLALIGIMLPPEGLPHVLLPYMCHGDLLQFIRSPQRNPTVKDLISFGLQVARGMEYLAEQKFVHRDLAARNCMLDESFTVKVADFGLARDILDREYYSVQQHRHARLPVKWMALESLQTYRFTTKSDVWSFGVLLWELLTRGAPPYRHIDPFDLTHFLAQGRRLPQPEYCPDSLYQVMQQCWEADPAVRP

• Full Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• Calculated Molecular Weight: 152 kDa
• Observed Molecular Weight: 150-185 kDa
• GenBank Accession Number: BQ936677 NM_002447
• Gene Symbol: RON
• Gene ID (NCBI): 4486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q04912
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.