RPL13 Polyclonal antibody

RPL13 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11271-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

60S ribosomal protein L13, BBC1, D16S444E, Large ribosomal subunit protein eL13, OK/SW-cl.46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, SGC-7901 cells, human lung tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11271-1-AP targets RPL13 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1814

Product name: Recombinant human RPL13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC014167

Sequence: MAPSRNGMVLKPHFHKDWQRRVATWFNQPARKIRRRKARQAKARRIAPRPASGPIRPIVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPRRRNKSTESLQANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNVYKKEKA

Predict reactive species
Full Name ribosomal protein L13
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC014167
Gene Symbol RPL13
Gene ID (NCBI) 6137
RRIDAB_2269735
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP26373
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].

Protocols

Product Specific Protocols
WB protocol for RPL13 antibody 11271-1-APDownload protocol
IHC protocol for RPL13 antibody 11271-1-APDownload protocol
IP protocol for RPL13 antibody 11271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
humanWB

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
mouseWB

Ecotoxicol Environ Saf

ZEA and DON inhibited inflammation after L. monocytogenes infection and induced ribosomal hyperfunction.

Authors - Guodong Cai
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
mouseWB

Adv Sci (Weinh)

m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control

Authors - Lisha Yin
mouseIF

Cell Rep

Single-nucleus profiling of mouse inner ear aging uncovers cell type heterogeneity and hair cell subtype-specific age-related signatures

Authors - Mingyu Xia
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